Journal: Journal of Neuroinflammation

Article Title: IL-37 suppresses CNS autoimmunity by increasing the frequency of Treg cells and reducing CD4 + T cell-derived IL-10 production

doi: 10.1186/s12974-024-03295-1

Figure Lengend Snippet: IL-37 treatment ameliorated EAE at both priming and ongoing phases. ( A ) C57BL/6 mice were immunized to induce EAE and treated i.p. with IL-37 (1 µg/ dose; n = 5) and PBS ( n = 4) at days 1, 4, and 7 p.i. The clinical scores were evaluated daily for 21 days p.i. Mean clinical scores for IL-37-treated and PBS-treated groups were calculated as the sum of daily scores for each mouse divided by the number of days per group. ( B-C ) EAE mice were treated i.p. with 1 µg of rhIL-37b/ day when they showed the first symptoms of EAE. PBS was administered i.p. to the control group in the same way. Clinical scores were followed for 20 days p.i. (peak; n = 7 per group) ( B ) and 26 days p.i. (chronic; n = 4 per group) ( C ), and mean clinical scores were compared between IL-37-treated mice and control. Clinical scores were analyzed by multiple unpaired t-tests. Mean clinical scores were analyzed by Student’s t-test. Data shown as mean ± SEM. Values of p < 0.05 (*), p < 0.01 (**) and p < 0.001 (***) were considered significant. Ns: not significant

Article Snippet: Recombinant human IL-37b (rhIL-37b) (R&D Systems) was reconstituted in phosphate-buffered saline (PBS) at 200 μg/mL.

Techniques: Control

Journal: Journal of Neuroinflammation

Article Title: IL-37 suppresses CNS autoimmunity by increasing the frequency of Treg cells and reducing CD4 + T cell-derived IL-10 production

doi: 10.1186/s12974-024-03295-1

Figure Lengend Snippet: IL-37 enhanced MBP expression. ( A ) The lumbar section of the spinal cord was harvested from EAE mice treated with PBS (left) or rhIL-37b (middle) at the peak of disease ( n = 4 mice in each group). Naïve mice (right) are illustrated as a control to show healthy spinal cord. ( B ) Mean fluorescent intensity of MBP expression in both groups was measured using FIJI software. Unpaired t-test was performed to analyze these two groups. Data shown as mean ± SEM. Values of p < 0.05 (*), p < 0.01 (**) and p < 0.001 (***) were considered significant. Ns: not significant

Article Snippet: Recombinant human IL-37b (rhIL-37b) (R&D Systems) was reconstituted in phosphate-buffered saline (PBS) at 200 μg/mL.

Techniques: Expressing, Control, Software

Journal: Nature Communications

Article Title: Protein degradation by human 20S proteasomes elucidates the interplay between peptide hydrolysis and splicing

doi: 10.1038/s41467-024-45339-3

Figure Lengend Snippet: Number and frequency of peptide products identified and quantified in the 15 protein digestions by inSPIRE 1.5 - aSPIRE

Article Snippet: The human 20S proteasomes used for the degradation of mature forms of human IL-1α, IL-1β and IL-37b were purchased from Enzo Life Sciences (BML-PW8720-0050; see Supplementary Fig. ).

Techniques: